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KMID : 1094720090140050606
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Biotechnology and Bioprocess Engineering
2009 Volume.14 No. 5 p.606 ~ p.611
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Bioethanol Production from Ammonia Percolated Wheat Straw
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Han Min-Hee
Moon Se-Kwon
Kim Yule
Kim Young-Ran
Chung Bong-Woo
Choi Gi-Wook
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Abstract
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This study examined the effectiveness of ammonia percolation pretreatment of wheat straw for ethanol production. Ground wheat straw at a 10% (w/v) loading was pretreated with a 15% (v/v) ammonia solution. The experiments were performed at treatment temperature of 50~170°C and residence time of 10~150 min. The solids treated with the ammonia solution showed high lignin degradation and sugar availability. The pretreated wheat straw was hydrolyzed by a cellulase complex (NS50013) and β-glucosidase (NS50010) at 45°C. After saccharification, Saccharomyces cerevisiae was added for fermentation. The incubator was rotated at 120 rpm at 35°C. As a result of the pretreatment, the delignification efficiency was > 70% (170°C, 30 min) and temperature was found to be a significant factor in the removal of lignin than the reaction time. In addition, the saccharification results showed an enzymatic digestibility of > 90% when 40 FPU/g cellulose was used. The ethanol concentration reached 24.15 g/L in 24 h. This paper reports a total process for bioethanol production from agricultural biomass and an efficient pretreatment of lignocellulosic material.
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KEYWORD
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bioethanol, ammonia percolation, wheat straw, enzymatic hydrolysis, fermentation
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